Senior Research Associate Job at Oregon Health & Science University
Department Overview:
The position requires that the candidate have experience with molecular biology: characterizing proteins using polyacrylamide gels and western blots, manipulating plasmid DNAs, cell culture and transfection of cells. Laboratory experience with manipulating and expressing plasmids and with mammalian cell culture and transfection of cells is essential. Experience with handling viruses such as HSV or other viruses will also be valuable. Experience with culturing neurons will also be very helpful. Applicants must have a BSc, although those with MSc or PhD degrees and experience will be given priority. Function/Duties of Position:
As an organization devoted to the health and well-being of people in Oregon and beyond, OHSU requires its employees to be fully vaccinated against COVID-19.
This Senior Research Associate position is in support of ongoing research projects in the Johnson lab. Research is focused on understanding how herpes simplex virus (HSV) proteins and virus particles are transported in neuronal axons. Involves manipulating and producing plasmid DNAs, which will be transfected into neurons cells to produce HSV proteins that are transported into neuronal axons. Other work will involve studies of protein-protein interactions to understand which HSV proteins interact with kinesin motors that transport virus particles in axons.
The position requires that the candidate have experience with molecular biology: characterizing proteins using polyacrylamide gels and western blots, manipulating plasmid DNAs, cell culture and transfection of cells. Laboratory experience with manipulating and expressing plasmids and with mammalian cell culture and transfection of cells is essential. Experience with handling viruses such as HSV or other viruses will also be valuable. Experience with culturing neurons will also be very helpful. Applicants must have a BSc, although those with MSc or PhD degrees and experience will be given priority.
- Supervising an RA1 who will order supplies, wash dishes, do routine maintenance in the lab and perform experiments.
- Producing virus stocks and using these viruses to infect neurons.
- Passaging and caring for neuronal cell lines and imaging these cells using confocal microscopy in order to determine how viruses and viral proteins are transported into neuronal axons.
- Studying interactions between viral proteins and kinesin motors using molecular biologic techniques.
- Manipulating and producing plasmid DNAs, which will be transfected into neurons to characterize protein transport into axons.
- Preparing representations of data and working with the PI to write papers and grant applications.
- PhD in relevant field AND 1 year of relevant experience OR
- Master's Degree in relevant field AND 5 years of relevant experience OR
- Bachelor's Degree in relevant field AND 9 years of relevant experience.
- Laboratory experience with manipulating and expressing plasmids and with mammalian cell culture and transfection of cells is required.
- Experience with transfecting cells, propagating viruses and culturing neurons will also be very valuable.
- Prior experience in a mentorship or leadership role.
Work Schedule:
full time days, location RJH, standard microbiology laboratory conditions, exposures such as hazardous chemicals and biohazard waste. Ability to perform common laboratory protocols and tissue culture work.
All are welcome: Oregon Health & Science University values a diverse and culturally competent workforce. We are proud of our commitment to being an equal opportunity, affirmative action organization that does not discriminate against applicants on the basis of any protected class status, including disability status and protected veteran status. Individuals with diverse backgrounds and those who promote diversity and a culture of inclusion are encouraged to apply. To request reasonable accommodation contact the Affirmative Action and Equal Opportunity Department at 503-494-5148 or aaeo@ohsu.edu.
As an organization devoted to the health and well-being of people in Oregon and beyond, OHSU requires its employees to be fully vaccinated against COVID-19.
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